Research

Nimesh Gupta

M. Sc.
Jiwaji University

Ph. D.
Jiwaji University

Past Affiliations
National Institute of Health and Medical Research, INSERM-UMRS-1138, Paris, France
Institute Pasteur, Paris, France

Email

nimesh.gupta@nii.ac.in


Research Interest

Our laboratory is interested in understanding the immunological determinants underlying the establishment of protective humoral immunity. The major focus of investigations is to resolve the traits and function of a specialized subset of CD4 T cells known as follicular T helper (Tfh) cells in long-term protective immunity. The global aim is to identify and harness the positive attributes of Tfh cells for rational design of vaccines.

Group Members

Anurag Kalia, Shilpa Sachan, Asgar Ansari, Bhushan Nikam, Someshwar Nath Jha (PhD scholars); Debarchana Saha, Jitendra Kumar (Postdoctoral fellows); Anupama Pai, Rajesh Vikkurthi (Project staff); Sudipta Das, Inderjit Singh (Technical officers)

Summary of Research

Our lab works at the interface of basic and translational immunology. By providing help to B cells, Tfh cells are crucial to the development of germinal center derived high quality protective antibodies. The factors that govern the differentiation of potent Tfh cells and the biology of Tfh cells implicated in the development of optimal humoral responses remain largely uncharacterized. Therefore, the conceptual framework of our programs is to resolve the traits and function of Tfh cells in long-term sustained immunity. Moreover, we are also trying to understand the characteristics of immunological memory, with emphasis on the T cells, to Flavivirus and human Coronavirus. To this, we are integrating the viral immunology, deep immunophenotyping, immunogenomics, and metabolomics approaches to investigate the biology of CD4 T cells and Tfh cells in two different models; (i) Human vaccination. Here, we are studying the characteristics and clonotypic diversity of human Tfh cells established in response to licensed human vaccines like SA14-14-2 Japanese encephalitis (JE) vaccine, SARS-CoV-2 vaccines etc. To identify the ideal attributes of Tfh cells in long-term protective immunity, we are comparing the function and traits of Tfh cells induced in response to vaccine with the Tfh cells induced in case of successful recovery from natural infection. To do this, we have established the longitudinal cohorts for controlled vaccination and infection. Besides, we are also using mouse models to mechanistically investigate the biology of Tfh cells in protective immunity established by the human vaccines. (ii) Human infection. In this program, our attempts are focused on providing the insight into the immunological memory and cellular determinants of humoral responses to viruses like Dengue virus, JEV, SARS-CoV-2 etc. Here, we are exploring the biology of Tfh cells and related CD4 T-cell subsets in patients with diverse outcome of virus infection. 

Extramural Funding

Department of Biotechnology, Department of Science and Technology (SERB), NER-BPMC Twinning Program (DBT), Ramalingaswami Fellowship, Indo-USA Human Immunophenotyping Consortium, National Biopharma Mission Dengue Consortium (BIRAC), National Biopharma Mission COVID-19 Consortium (DBT-BIRAC), Intensification of Research in High Priority Areas (DST). 

Awards/Fellowships

  • Young Scientist award, India International Science festival-2020 (2021)
  • Early Career Faculty Award by American Association of Immunology (2020)
  • The Merieux Foundation Fellowship for the Advancement in Vaccinology (2019)
  • Martin Villar Award (2016)
  • Ramalingaswami Fellowship (2016)
  • Global Health Travel Award of Bill and Melinda Gates Foundation (2016)
  • European Federation of Immunological Societies Young Investigator Award (2013).
  • International Society of Thrombosis & Haemostasis Young Investigator Award (2013).
  • Raman-Charpak Fellowship, Institute Pasteur, Paris, France (2008).
  • Brig. K.M. Rao Award, Defence R&D Establishment, India (2008-2010).

Selected Publications

  • Asgar Ansari, Rakesh Arya, Shilpa Sachan, Someshwar Nath Jha, Anurag Kalia, Anupam Lall, Alessandro Sette, Alba Grifoni, Daniela Weiskopf, Poonam Coshic, Ashok Sharma, and Nimesh Gupta (2021) Immune memory in mild COVID-19 patients and unexposed donors reveals persistent T cell responses after SARS-CoV-2 infection. Frontiers in Immunology 12:636768.
  • Alain Townsend, Pramila Rijal, Julie Xiao, Tiong Kit Tan, Kuan-Ying A Huang, Lisa Schimanski, Jiangdong Huo, Nimesh Gupta, ….. Etienne Joly (2021) A haemagglutination test for rapid detection of antibodies to SARS-CoV-2. Nature Communication
  • Anurag Kalia, Mona Agrawal and Nimesh Gupta (2020) CD8+ T cells are crucial for humoral immunity establishment by SA14-14-2 live attenuated Japanese encephalitis vaccine. European Journal of Immunology. 10.1002/eji.202048745.
  • Gupta N, Culina S, Meslier Y, Dimitrov J, Delignat S, Gangadharan B, Lecerf M, Justesen S, Salomon B, Scott DW, Kaveri SV, Mallone R, Lacroix-Desmazes S (2015) Prevention of immune responses to antigens and protein therapeutics by transplacental induction of central and peripheral T-cell tolerance. Science Trans Med. 7: 275ra21.
  • Gupta N*, Wispelaere M, Lecerf M, Kalia M, Scheel T, Vrati S, Berek C, Kaveri S, Despres P, Lacroix-Desmazes S, Dimitrov J (2015) Neutralization of Japanese Encephalitis Virus by heme induced broadly reactive human monoclonal antibody. Scientific Reports. 5, 16248. *Corresponding Author
  • Culina S, Gupta N, Boisgard R, Afonso G, Gagnerault MC, Dimitrov J, Østerbye T, Justesen S, Luce S, Attias M, Kyewski B, Buus S, Wong FS, Lacroix-Desmazes S, Mallone R (2015) Materno-fetal transfer of preproinsulin through the neonatal Fc receptor prevents autoimmune diabetes. Diabetes. pii: db150024
  • Gupta N, Hegde P, Lecerf M, Nain M, Kalia M, Vrati S, Bayry J, Lacroix-Desmazes S, Kaveri S (2014) Japanese encephalitis virus expands regulatory T cells by increasing the expression of PD-L1 on dendritic cells. Eur J Immunol. 44:1363-1374.
  • Gupta N, Legoff J, Chamat S, Mercier-Delarue S, Touzelet O, Power UF, Kazatchkine MD, Simon F, Lacroix-Desmazes S, Bayry J, Kaveri S (2013) Affinity-purified Respiratory Syncytial virus antibodies from intravenous immunoglobulin exert potent antibody-dependent cellular cytotoxicity. PLoS ONE. 8(7): e69390.
  • Gupta N and Lakshmana Rao PV (2011) Transcriptomic profile of host response in Japanese encephalitis virus infection. Virol J. 8:92.
  • Gupta N, Bhaskar AS, and Lakshmana Rao PV (2011) Transcriptional regulation and activation of the mitogen-activated protein kinase pathway after Japanese encephalitis virus infection in neuroblastoma cells. FEMS Immunol Med Microbiol. 62:110-121.
  • Ravindran J, Gupta N, Agrawal M, Bala Bhaskar AS, and Lakshmana Rao PV (2011) Modulation of ROS/MAPK signaling pathways by okadaic acid leads to cell death via, mitochondrial mediated caspase-dependent mechanism. Apoptosis. 16:145-161.
  • Gupta N, Lomash V, and Lakshmana Rao PV (2010) Expression profile of Japanese encephalitis virus induced neuroinflammation and its implication in disease severity. J Clin Virol. 49:4-10.
  • Gupta N, Santhosh SR, Babu JP, Parida MM, and Lakshmana Rao PV (2010) Chemokine profiling of Japanese encephalitis virus-infected mouse neuroblastoma cells by microarray and real-time RT-PCR: implication in neuropathogenesis. Virus Res. 147:107-112.

Patents
WO2017/012959: Methods and pharmaceutical compositions for inducing immune tolerance by mucosal vaccination with Fc-coupled antigens.
US62/188,004: Prenatal therapy to induce immune tolerance.

Copyright © 2012 National Institute of Immunology. All Rights Reserved.

150 Years of Celebrating The Mahatma