National Institute of Health and Medical Research, INSERM-UMRS-1138, Paris, France
Institute Pasteur, Paris, France
Harnessing follicular CD4 T helper (TFH) cells for rationalizing vaccine development.
Anurag Kalia, Shilpa Sachan, Mona Agrawal, Vishal Gupta
Summary of Research
The Vaccine Immunology Laboratory works at the interface of basic and clinical research to fast-track the translation of our team efforts from bench to clinic. The ultimate aim is to identify novel approaches for efficient vaccination by investigating, and adapting the favorable response of TFH cells in protective antiviral immunity. TFH cells are the uniquely specialized T-cell subset providing help to B-cells. TFH cells are, therefore, limiting for the magnitude of germinal centers and germinal center derived long-term protective antibody responses. Illumination of the TFH-cell biology and their function during the tide of an immune response is thus indispensable for the rational development of efficient vaccines.
At present by integrating multidisciplinary approaches we are investigating the TFH cells in two models; (i) human dengue infection and (ii) flavivirus encephalitis vaccine. In our first model system, to address our hypothesis, the workflow utilizes human dendritic cell cultures and samples from dengue-infected individuals. The program on flavivirus encephalitis vaccine covers the investigations in mutant mouse models and the human vaccinees.
- Martin Villar Award (2016)
- Ramalingaswami Fellowship (2016)
- Global Health Travel Award of Bill and Melinda Gates Foundation (2016)
- European Federation of Immunological Societies Young Investigator Award (2013).
- International Society of Thrombosis & Haemostasis Young Investigator Award (2013).
- Raman-Charpak Fellowship, Institute Pasteur, Paris, France (2008).
- Brig. K.M. Rao Award, Defence R&D Establishment, India (2008-2010).
- Gupta N, Culina S, Meslier Y, Dimitrov J, Delignat S, Gangadharan B, Lecerf M, Justesen S, Salomon B, Scott DW, Kaveri SV, Mallone R, Lacroix-Desmazes S (2015) Prevention of immune responses to antigens and protein therapeutics by transplacental induction of central and peripheral T-cell tolerance. Science Trans Med. 7: 275ra21.
- Gupta N*, Wispelaere M, Lecerf M, Kalia M, Scheel T, Vrati S, Berek C, Kaveri S, Despres P, Lacroix-Desmazes S, Dimitrov J (2015) Neutralization of Japanese Encephalitis Virus by heme induced broadly reactive human monoclonal antibody. Scientific Reports. 5, 16248. *Corresponding Author
- Culina S, Gupta N, Boisgard R, Afonso G, Gagnerault MC, Dimitrov J, Østerbye T, Justesen S, Luce S, Attias M, Kyewski B, Buus S, Wong FS, Lacroix-Desmazes S, Mallone R (2015) Materno-fetal transfer of preproinsulin through the neonatal Fc receptor prevents autoimmune diabetes. Diabetes. pii: db150024
- Gupta N, Hegde P, Lecerf M, Nain M, Kalia M, Vrati S, Bayry J, Lacroix-Desmazes S, Kaveri S (2014) Japanese encephalitis virus expands regulatory T cells by increasing the expression of PD-L1 on dendritic cells. Eur J Immunol. 44:1363-1374.
- Gupta N, Legoff J, Chamat S, Mercier-Delarue S, Touzelet O, Power UF, Kazatchkine MD, Simon F, Lacroix-Desmazes S, Bayry J, Kaveri S (2013) Affinity-purified Respiratory Syncytial virus antibodies from intravenous immunoglobulin exert potent antibody-dependent cellular cytotoxicity. PLoS ONE. 8(7): e69390.
- Gupta N and Lakshmana Rao PV (2011) Transcriptomic profile of host response in Japanese encephalitis virus infection. Virol J. 8:92.
- Gupta N, Bhaskar AS, and Lakshmana Rao PV (2011) Transcriptional regulation and activation of the mitogen-activated protein kinase pathway after Japanese encephalitis virus infection in neuroblastoma cells. FEMS Immunol Med Microbiol. 62:110-121.
- Ravindran J, Gupta N, Agrawal M, Bala Bhaskar AS, and Lakshmana Rao PV (2011) Modulation of ROS/MAPK signaling pathways by okadaic acid leads to cell death via, mitochondrial mediated caspase-dependent mechanism. Apoptosis. 16:145-161.
- Gupta N, Lomash V, and Lakshmana Rao PV (2010) Expression profile of Japanese encephalitis virus induced neuroinflammation and its implication in disease severity. J Clin Virol. 49:4-10.
- Gupta N, Santhosh SR, Babu JP, Parida MM, and Lakshmana Rao PV (2010) Chemokine profiling of Japanese encephalitis virus-infected mouse neuroblastoma cells by microarray and real-time RT-PCR: implication in neuropathogenesis. Virus Res. 147:107-112.
- Verma SK, Kumar S, Gupta N, Vedi S, Bhattacharya SM, and Lakshmana Rao PV (2009) Bacterially expressed recombinant envelope protein domain III of Japanese encephalitis virus (rJEV-DIII) elicits Th1 type of immune response in BALB/c mice. Vaccine. 27:6905-6909.
- Babu JP, Pattnaik P, Gupta N, Shrivastava A, Khan M, and Lakshmana Rao PV (2008) Immunogenicity of a recombinant envelope domain III protein of dengue virus type-4 with various adjuvants in mice. Vaccine. 26:4655-4663.
WO2017/012959: Methods and pharmaceutical compositions for inducing immune tolerance by mucosal vaccination with Fc-coupled antigens.
US62/188,004: Prenatal therapy to induce immune tolerance.