Research

Mass Spectrometer

The Central Mass Spec Facility (CMSF) of NII provides support and services for protein identification and characterization. Core activities include identification of proteins and determination of post -translational modification using state of art mass spectrometry. Following are the equipments at the CMSF.

  • Mass Spectrometers: Thermo LTQ ORBITRAP VELOS
  • HPLC: Proxeon EASY nano-LC 1200
  • MS Data Analysis Softwares: Proteome Discover 2.2, GPS Explorer 3.6, Progenesis, Byonic

Thermo LTQ ORBITRAP VELOS: NII had procured Thermo LTQ Velos HR-MS with Proxeon nano-LC in the year 2010 and the installation was completed by March 2011. Once the machine became functional, operating demo and introductory training of Orbitrap with proxeon nano-LC was provided to NII research scholars from different labs. For data acquisition various labs are using Orbitrap with nano-LC. Routine calibration and maintenance for instrument is done. The instrument is mostly used for both the qualitative and quantitative proteomics as well as identification of post-translational modifications. Analysis is performed using Thermo proteomic discoverer software. Various methods are under development to increase proteome coverage.

For using the above infrastructure please click here to download the form

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