Research

Confocal Microscopy Facility

The Zeiss LSM 510 META system is the multiple award winning flagship of the Zeiss microscope range. The LSM 510 META is an extremely versatile laser scanning confocal microscope system available in both an upright or inverted format.

The confocal system in National Institute of Immunology (NII) is a Zeiss LSM 510 META system. It consists of an inverted microscope, fluorescence system, camera, confocal module with visible lasers and software. The inverted microscope consists of a motorized microscope stand with motorized nosepiece, motorized FL filter wheel, motorized focus, automatic shutters for Bright field, DIC and FL illumination techniques and integrated 12V-100W power source for transmitted light applications. The apochromatic objectives present are 10X/0.4, 20X/0.7, 40X /0.85 Korr with DIC attachment and 63X /1.4 Oil with DIC. The visible laser sources are Red (HeNe 633nm/5mW), DPSS (561nm/ 10mW), HeNe (594nm/5mW), Blue (Ar 458nm/5mW; 477nm/5mW; 488nm/20mW; 514nm/30mW). The computer attached with the system is Pentium-4, 3 GHz with hyper threading. It has 160GB HDD, 1 GB RAM, DVD+R/RW drive, Windows XP Professional.

The acquisition and image analysis is done via the Zeiss LSM 5 image browser. This browser not only produces a library of the images taken, but records important data such as the scan mode, lasers, objectives and filters used, the pinhole and stack size for each image. Notes can be assigned to each image and multiple images can be saved with the same name. The software also allows the user to customize the image, such as assigning colors to each channel before exporting the image.

At present three colour imaging on fixed cell culture samples are being offered.

For using the above infrastructure please click here to download the form

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