Research

Arnab Mukhopadhyay

M. Sc.
University of Delhi, India

Ph. D.
University of Delhi, India

Postdoctoral Research
University of Massachusetts Medical School, 
Worcester, MA, USA.

Email
arnab@nii.ac.in

 

Research Interest

Longevity, Dietary restriction, Insulin-IGF-1 signalling pathway, metabolism, innate immunity, Genomics, Next generation sequencing

Group Members

Anupama Singh, Shamsh Tabrez, Sonia Verma, Anita Goyala, Amit Garg, Gautam Sarkar, Vaibhav Jain, Sonu Gupta

Summary of Research

The primary focus of my lab is to understand the molecular mechanisms that determine the longevity of an organism. The main interests of the lab are:

a) The biology of Dietary restriction
b) Deciphering the molecular links between aging and innate immunity
c) Regulation of the Insulin-IGF-1 signaling pathway
d) Screening for novel compounds that extend longevity

We use a combination of molecular biology, genomics, Next Generation sequencing and molecular genetics approaches in the lab. Caenorhabditis elegans is the system of choice for our studies due to its short and reproducible life span, low maintenance cost, amenability to high throughput molecular techniques as well as ease of genetic manipulation. Interestingly, the molecular makeup of the innate immune response pathways and those that regulate longevity are strikingly similar between vertebrates and invertebrates. Such conservations are a key to extrapolating the results obtained in our model system and validating them in mammals.

Awards / Fellowships

Ramalingaswami Fellowship (2009-14)

Selected Publications

  • Maity S*, Rajkumar A*, Matai L*, Bhat A, Ghosh A, Agam G, Kaur S, Bhatt NR, Mukhopadhyay A^, Sengupta S^, Chakraborty C^ (2016) Oxidative homeostasis regulates response to reductive Endoplasmic Reticulum stress through translation control. Cell Reports (in press). *Co-first author, ^Equally contributing senior authors 
  • Singh A, Kumar N, Matai L, Garg A and Mukhopadhyay A (2016) A chromatin modifier integrates insulin/IGF-1 signalling and dietary restriction to regulate longevity. Aging Cell (in press)
  • Kumar N^, Jain V*, Singh A*, Jagtap U, Verma S and Mukhopadhyay A^ (2015) Genome-wide endogenous DAF-16/FOXO recruitment dynamics during lowered insulin signalling in C. elegans. Oncotarget  6(39):41418-33. *Equal contributions, ^Corresponding authors
  • Golegaonkar S*, Tabrez SS*, Pandit A, Shalini S, JagadeeshaPrasad MG, Bansode S, Sampathkumar SG, Kulkarni MJ^ and Mukhopadhyay A.^ (2015) Rifampicin reduces Advanced Glycation End products and activates DAF-16 to increase life span in Caenorhabditis elegans. Aging Cell 14(3):463-73. doi: 10.1111/acel.12327.  *Co-first authors, ^Corresponding authors
  • Pandit A, Jain V, Kumar N, and Mukhopadhyay A. (2014) PHA‐4/FOXA‐regulated microRNA feed forward loops during Caenorhabditis elegans dietary restriction. Aging 6(10): 835-851.
  • Chamoli M, Singh A, Malik Y, Mukhopadhyay A. (2014) A novel kinase regulates Dietary Restriction-mediated longevity in C. elegans. Aging Cell 13(4):641-55. doi: 10.1111/acel.12218.
  • Ritter AD, Shen Y, Fuxman Bass J, Jeyaraj S, Deplancke B, Mukhopadhyay A, Xu J, Driscoll M, Tissenbaum HA, Walhout AJ. (2013) Complex expression dynamics and robustness in C. elegans insulin networks. Genome Res. 23(6):954-65.
  • Parker JA, Vazquez-Manrique RP, Tourette C, Farina F, Offner N, Mukhopadhyay A, Orfila AM, Darbois A, Menet S, Tissenbaum HA, Neri C. (2012) Integration of ß-catenin, sirtuin, and FOXO signaling protects from mutant huntingtin toxicity.  J Neurosci 32:12630-40.
  • Padmanabhan S*, Mukhopadhyay A*, Narasimhan S*, Tesz G, Czech MP, Tissenbaum HA (2009) A PP2A regulatory subunit, PPTR-1, regulates C. elegans insulin signaling by modulating AKT-1 phosphorylation. Cell1 36:939-951.*Co-first author paper
  • Mukhopadhyay A*, Deplancke B*, Walhout AJM, Tissenbaum HA (2008) Chromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA inCaenorhabditis elegans. Nature Protocols 3:698-709. *Co-first author paper
  • Mukhopadhyay A, Pan X, Lambright D, Tissenbaum HA (2007) An endocytic pathway as a target of tubby for regulation of fat storage. EMBO Reports 8:931-938.
  • Deplancke B, Mukhopadhyay A,  Ao W, Elewa AM, Grove CA, Martinez NJ, Sequerra R, Doucette-Stamm L, Tissenbaum HA, Mango SE, Walhout AJM (2006) A gene-centered C. elegans protein-DNA interaction network. Cell 125:1193-205.
  • Oh SW*, Mukhopadhyay A*, Dixit BL, Raha T, Green MR, Tissenbaum HA (2006) Identification of direct DAF-16 targets controlling longevity, metabolism and diapause by chromatin immunoprecipitation. Nature Genetics 38:251-257. *Co-first author paper
  • Mukhopadhyay A, Deplancke B, Walhout AJM, Tissenbaum HA (2005) C. elegans tubby regulates life span and fat storage by two independent mechanisms. Cell Metabolism 2:35-41.
  • Oh SW, Mukhopadhyay A, Svrzikapa N, Jiang F, Davis RJ, Tissenbaum HA (2005) JNK regulates life span in C. elegans by modulating nuclear translocation of FOXO/DAF-16. Proceedings National Academy of Sciences USA 102:4494-4499.

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