Research

Amulya K. Panda

M. Tech.
Indian Institute of Technology, Madras, India

Ph. D. 
Indian Institute of Technology, New Delhi, India

Visiting Scientist
University of California at Berkeley, USA; Nebraska Medical Center,Omaha,  USA

Email
amulya@nii.res.in

 

Research Interest

Application of chemical enginerring priciples to biological process . This  includes developmemsnt of  high cell density fed-batch fermenation fermentaion process for production nof recombinant proteins, high throughput protein refolding from inlcusion bodies and design of  vaccines delivery system using  biodegrdable polymer particles.

Group Members

Vaibhav Upadhyay, Jairam Meena,  Divya Jha, Robin Kumar & Akansha Singh. Dr. Debasis Sahu (DST Scientist) 

Summary of Research

(A) Fermentation   research experiences on Yeast, Fungus, Plant Cells, Animal Cells, Insect Cells, Hybridoma, Viruses and recombinant E. coli for the production of biochemicals.  Developed a novel high cell density fermentation process for production of growth hormone in E. coli (Indian patent granted and   J. Biotechnology, 1999).  

(B) High throughput refolding and purification of   recombinant proteins from E. coli. Developed novel ways of protein refolding from inclusion bodies of E. coli without using high molar concentration of chaotropic salts. Mild inclusion body protein solubilization process has been granted USA patent and the research paper published on the novel method (J. of Bioscience and Bioengineering, 2005) has been cited nearly 450 times till date as per Google Scholar. 

(C) Development of vaccine formulation using biodegradable polymer particles. This involves formulation of different size polymeric particles for improved immunogenicity of candidate vaccines. Six patent applications have been filed on novel applications of  polymer particles based vaccine delivery systems. We have shown nthatr use of biodegradable polymer particle leads to improved immunological memory from single point application. Currently exploring delivery of carbohydrate based vaccine and biomolecules using biodegradable polymer particles.

Achievemnets 

Having a master degree in chemical engineering from IIT Madras on 1986, I have been working on different areas of bioprocess engineering for last 30 years. I have experiences on all the three areas on biological manufacturing namely fermentation, downstream processing and formulation of biologicals. I do innovative bioprocess engineering utilizing basic fundamental rules of biology. I have significantly contributed towards for growth of plant cells in bioreactor for the production of secondary metabolites (1991) and have reported the very first study on high cell density recombinant fermentation from India (1995). I have reported the highest amount of recombinant protein expression among the earliest Indian studies (1999). Novel protein refolding methods developed by me has been accepted worldwide for high throughput recovery of bioactive protein from inclusion bodies of E. coli (2005). Because of my contribution on applications of chemical engineering principles to biological processes, I have been awarded the Young Asian Biotechnologist Prize from the Society for Biotechnology, Japan, for the year 2004. I have also developed innovative applications of polymeric particles based delivery system for generation of memory antibody response from single doses (2009) and have reported the generation of memory antibody response against carbohydrate antigens without conjugating them to a carrier protein (2014). I have the credentials of generating highest number of IPRs while working at National Institute of Immunology.

Total Research Publications: 100, Patents: 25
Research Presentations: 65, Book Chapters: 9
Technologies Developed: 12, Trademark Products: 1 

Awards / Fellowships

  • Dr. GB Manjerkar Award of the Association of Microbiologist of India (2010)
  • Tata Innovation Fellowship, Dept. of Biotechnology, Govt. of India (2009-2010)
  • Research group awarded 3rd prize at the World Innovation Summit at Barcelona, Spain (2009)
  • Research Group awarded 2nd prize at the Intel-UC Berkeley, Technology Entrepreneurship Challenge, UC Berkeley, USA (2008)
  • Awarded Young Asian Biotechnologist Prize from The Society for Biotechnology, Japan (2004)
  • Awarded   DBT Overseas Associateship to work at   Nebraska Medical Center, Omaha, USA (2002-2003)
  • Awarded   Excellence in Scientific Achievements, from National Institute of Immunology, New Delhi (2001)
  • Biotech Product and Process Development and Commercialization Award by the  Dept. of Biotechnology, Ministry of Science and Technology, Govt. of India (2001)
  • Awarded “SAMANTA CHANDRASEKHARA AWARD”, Orissa Bigyan Academy (2000).
  • Award for the “Best scientific work leading to a product”  from National Institute of Immunology, New Delhi (1990)

Selected Publications

  • Dende C, Meena J, Nagarajan P, Panda AK, Rangarajan PN,  Padmanaban G. (2015) Sci Rep. 2015 Jul 31;5:12671.
  • Anish CK, Khan N, Upadhyay AK, Sehgal D, Panda AK.(2014) Mol Pharmaceutics 11: 922-937
  • Anish CK, Goswami DG, Kanchan V, Mathew S and Panda AK (2012) Biomaterials 33: 6843-57.
  • Arun K. Upadhyay, Aruna Murmu, Anupam Singh and Amulya K.  Panda (2012) PLoS ONE 7(3):e33951.
  • A. K Panda (2011) Expert Rev Vaccines 10:155-157.
  • Vibhu Kanchan, Yogesh K. Katare and Amulya K. Panda (2009)  Biomaterials 30: 4763-4776.
  • Kanchan V and AK Panda (2007) Biomaterials 28: 5344-57.
  • S. M.  Singh and  Amulya K Panda (2005)  Journal of Biosciences and Bioengineering 99: 303-310..
  • Dhiman H. K, Alok R Ray and Amulya K Panda (2005) Biomaterials 26: 979-986.
  • A. K. Panda., R. H. Khan, K. B. C. Appa Rao, and S. M. Totey (1999) J. of Biotechnology 75: 161-172.
  • Virendra Bisaria and Amulya Panda (1991) Current Opinion in Biotechnology 2: 370-374.

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